Dutch Plus Test 

Comprehensive Hormone & Stress Response Test

The DUTCH Plus® (Dried Urine Test for Comprehensive Hormones) provides a complete evaluation of sex hormones, adrenal hormones, and their metabolites, offering detailed insight into how your body produces, metabolizes, and balances hormones.

In addition, this test measures the Cortisol Awakening Response (CAR)—a key biomarker that reflects how your body adapts to daily stress and the function of the HPA Axis (hypothalamus–pituitary–adrenal system).

The DUTCH Plus® uses four dried urine samples and five saliva samples collected throughout the day, from waking to bedtime, giving a clear picture of hormonal rhythm and metabolic balance.

What the DUTCH Plus® Analyzes

Sex Hormones & Metabolites

• Estrogens (E1, E2, E3) + hydroxylated / methoxylated metabolites

• Progesterone metabolites (α-pregnanediol, β-pregnanediol)

• Androgens (testosterone, DHEA-S, DHT, androstanediols, etc.)

Adrenal Function

• Free cortisol & cortisone (saliva — diurnal rhythm)

• Cortisol metabolites (urine)

• Total cortisol production

• Cortisol Awakening Response (CAR)

Additional Markers

• Organic acids (OATs)

• Melatonin metabolite (6-OHMS)

• Oxidative stress markers

Who Is It For?

Indicated for women and men experiencing:

• Fatigue or low energy

• Mood changes or anxiety

• Sleep disturbances

• Weight gain or resistance to weight loss

• Hair thinning

• Low libido

• Irregular cycles, PCOS, PMS, or fertility challenges

Not recommended for individuals under 12 years old.